Analysis Note

ControlMouse brain microsomal tissue lysate

Application

Immunocytochemistry Analysis: A previous lot of this antibody was used successfully by an independent laboratory in IC. (Pirotte, D., et al.)

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Neuroscience

Detect Neuregulin-1 using this Anti-Neuregulin-1 Antibody, clone D11 validated for use in WB & IC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The group of Neuregulin-1 proteins are cell-cell signaling molecules and are ligands for receptor tyrosine kinases of the ErbB/HER subfamily. Type III NRG1 proteins (also referred to as CRD-NRG1 proteins) play a critical role in neural development, including myelination, motor and sensory neuron survival, and neuromuscular synapse development (Falls, 2003). Defects in animals lacking type III NRGs include retraction of nerve terminals from newly formed synapses, absence of Schwann cells from peripheral nerves, and loss of motor and sensory neurons (Wolpowitz et al., 2000). Available evidence indicates that most Type III NRG1 proteins are synthesized as transmembrane proteins with 2 TM domains, and that proteolytic processing produces a bioactive transmembrane form (NTFm) which serves as a juxtacrine signal (Wang, 2001; Falls, 2003).

Immunogen

Epitope: Extracellular domain

His-tagged recombinant protein corresponding to mouse Neuregulin-1 at the extracellular domain.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G

Format: Purified

Quality

Evaluated by Western Blot in mouse brain microsomal tissue lysate.

Western Blot Analysis: 0.25 µg/mL of this antibody detected Neuregulin-1 on 10 µg of mouse brain microsomal tissue lysate.

Specificity

This antibody recognizes Neuregulin-1 at the extracellular domain.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~29 kDa observed. This target has been shown to undergo differential splicing during development into ~30 different isoforms.